Antimicrobial Susceptibility Pattern and Virulence Genes Detection in Citrobacter freundii Isolated from Patients of a Tertiary Care Hospital, Bangladesh

Background:
 Citrobacter freundii is an infrequent hospital acquired pathogen to cause different types of disease in clinical settings. This associated with wide range infections causing unpredictably high mortality rate 30-60%. Separation this health care settings escalating and multidrug resistant strains are emerging. Therefore, study aimed detect antimicrobial resistance pattern virulence genes among the isolated C. freundii.
 Methods:
 A total 500 samples (urine, stool, wound swab & pus, blood endotracheal aspirates sputum) from patients clinically suspected irrespective age sex were used study. Disc diffusion method was susceptibility antibiotics; colistin, tigecycline fosfomycin identified by minimum inhibitory concentration (MIC) method. Polymerase chain reaction (PCR) done potential genes.
 Results:
 Among 27 freundii, majority amoxiclav (92.59%), trimethoprim-sulfomethoxazole (88.89%), cefotaxime ceftazidime (85.19%) followed ceftriaxone (81.48%), cefepime ciprofloxacin (77.78%). MIC showed least colistin (29.63%), (11.11%) (7.41%). PCR positive for via B gene (48.15%) lt-A (25.93%). hly A, lt, lt-h negative results.
 Conclusion:
 Antibiotic found quite worrisome as widespread seen bacteria human infection. Also, play important role